Application
Antibody used in WB on Human HepG2 at 5.0 ug/ml.| Product Name | YWHAZ Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | YWHAZ, KCIP-1, MGC111427, MGC126532, MGC138156, HEL4, YWHAD, HEL-S-3, 14-3-3-zeta |
| Product description | YWHAZ belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity.This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene.1) Coblitz, B., (2005) J. Biol. Chem. 280 (43), 36263-36272. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human YWHAZ. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| YWHAZ antibody can be used for detection of YWHAZ by ELISA at 1:62500. YWHAZ antibody can be used for detection of YWHAZ by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | YWHAZ |
|---|---|
| Alternative Names | YWHAZ KCIP-1 MGC111427 MGC126532 MGC138156 HEL4 YWHAD HEL-S-3 14-3-3-zeta |
| Molecular Weight(MW) | 28 kDa, 28 kDa |
Application
Antibody used in WB on Human HepG2 at 5.0 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | YWHAZ antibody can be used for detection of YWHAZ by ELISA at 1:62500. YWHAZ antibody can be used for detection of YWHAZ by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store YWHAZ antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
