Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with 14-3-3 protein zeta/delta antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of hYWHAZ-D231 in mouse brain tissue and MCF-7 cell line lysates (35ug/lane)| Product Name | YWHAZ Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ |
| Product description | YWHAZ belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for the gene. Both variants encode the same protein, however, they are differentially expressed in hematopoietic cells.1) Powell, D.W., et al., Mol. Cell. Biol. 23(15):5376-5387 (2003). |
| Immunogen | This YWHAZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-245 amino acids from human YWHAZ. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | YWHAZ |
|---|---|
| Alternative Names | 14-3-3 protein zeta/delta Protein kinase C inhibitor protein 1 KCIP-1 YWHAZ |
| Molecular Weight(MW) | 28 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with 14-3-3 protein zeta/delta antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of hYWHAZ-D231 in mouse brain tissue and MCF-7 cell line lysates (35ug/lane)| Application Notes | For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
