Application
Western Blot at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: C2C12 whole cell lysate Lane 3: C6 whole cell lysate Lane 4: Daudi whole cell lysate Lane 5: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis in 293,HL-60,SiHa,K562,THP-1,U266 cell line lysates (35ug/lane).| Product Name | YY1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Transcriptional repressor protein YY1, Delta transcription factor, INO80 complex subunit S, NF-E1, Yin and yang 1, YY-1, YY1, INO80S |
| Product description | YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.1) Sucharov, C.C., et al., J. Biol. Chem. 278(33):31233-31239 (2003). Kurisaki, K., et al., Mol. Cell. Biol. 23(13):4494-4510 (2003). Huang, N.E., et al., Biochem. Biophys. Res. Commun. 306(1):267-275 (2003). Moriuchi, M., et al., J. Biol. Chem. 278(15):13003-13007 (2003). Hiromura, M., et al., J. Biol. Chem. 278(16):14046-14052 (2003). |
| Immunogen | This YY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-104 amino acids from the N-terminal region of human YY1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | YY1 |
|---|---|
| Alternative Names | Transcriptional repressor protein YY1 Delta transcription factor INO80 complex subunit S NF-E1 Yin and yang 1 YY-1 YY1 INO80S |
| Molecular Weight(MW) | 45 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: C2C12 whole cell lysate Lane 3: C6 whole cell lysate Lane 4: Daudi whole cell lysate Lane 5: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis in 293,HL-60,SiHa,K562,THP-1,U266 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
