Application
Western blot analysis of lysed extracts from HL60 cells using Zap-70 (Ab-319).Application
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Zap-70 (Ab-319).| Product Name | Zap 70 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SRK, STD, TZK, STCD, ZAP-70, NFKB3, 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZA70 |
| Product description | ZAP70 is a 70-kD tyrosine phosphoprotein that associates with the ζ chain and undergoes tyrosine phosphorylation following TCR stimulation. The ZAP70 gene is expressed in T- and natural KILLER cells. Protein-Tyrosine Kinases (PTKs) play an integral role in T-cell activation. Stimulation of the T-cell antigen receptor results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR-ζ chain, which can mediate the transduction of extracellular stimuli into cellular effector functions1) Salomon AR, et al. (2003) Proc Natl Acad Sci U S A; 100 (2): 443-448Ku GM, et al. (2001) EMBO J; 20 (3): 457-465Tang J, et al. (1999) Proc Natl Acad Sci U S A; 96 (17): 9775-9780Zhao Q, et al. (1996) Mol Cell Biol; 16 (12): 6765-6774Williams BL, et al. (1999) EMBO J; 18 (7): 1832-1844Adjali O, et al. (2005) J Clin Invest; 115 (8): 2287-2295. |
| Immunogen | Zap-70 (Ab-319) antibody was raised against a peptide sequence around aa.317~321 (S-P-Y-S-D) derived from Human ZAP70. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZAP70 |
|---|---|
| Alternative Names | SRK STD TZK STCD ZAP-70 NFKB3 70 kDa zeta-associated protein SRK Syk-related tyrosine kinase ZA70 |
| Molecular Weight(MW) | 70 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Zap-70 protein. |
| Entrez Gene | 7535 |
|---|---|
| Protein Accession | NP_001070.2 |
Application
Western blot analysis of lysed extracts from HL60 cells using Zap-70 (Ab-319).
Application
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Zap-70 (Ab-319).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
