Application
Western blot analysis of lysed extracts from HL60 cells using Zap-70 (Ab-493).Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Zap-70 (Ab-493).| Product Name | Zap 70 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SRK, STD, TZK, STCD, ZAP-70, ERBB, ERBB1, HER1, 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZA70 |
| Product description | ZAP70 is a 70-kD tyrosine phosphoprotein that associates with the ζ chain and undergoes tyrosine phosphorylation following TCR stimulation. The ZAP70 gene is expressed in T- and natural KILLER cells. Protein-Tyrosine Kinases (PTKs) play an integral role in T-cell activation. Stimulation of the T-cell antigen receptor results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR-ζ chain, which can mediate the transduction of extracellular stimuli into cellular effector functions1) Chan AC, et al. (1995) EMBO J; 14 (11): 2499-2508Kong G, et al. (1996) Mol Cell Biol; 16 (9): 5026-5035Salomon AR, et al. (2003) Proc Natl Acad Sci U S A; 100 (2): 443-448 |
| Immunogen | Zap-70 (Ab-493) antibody was raised against a peptide sequence around aa.491~495 (S-Y-Y-T-A) derived from Human ZAP70. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZAP70 |
|---|---|
| Alternative Names | SRK STD TZK STCD ZAP-70 ERBB ERBB1 HER1 70 kDa zeta-associated protein SRK Syk-related tyrosine kinase ZA70 |
| Molecular Weight(MW) | 70 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Zap-70 protein. |
| Entrez Gene | 7535 |
|---|---|
| Protein Accession | NP_001070.2 |
Application
Western blot analysis of lysed extracts from HL60 cells using Zap-70 (Ab-493).
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Zap-70 (Ab-493).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
