Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).Application
Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.Application
ZAP70 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | ZAP70 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.1) Zanotti, R., et al. Am. J. Hematol. 85(7):494-498(2010) |
| Immunogen | This ZAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-304 amino acids from the Central region of human ZAP70. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACS,IHC-P,WB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZAP70 |
|---|---|
| Alternative Names | Tyrosine-protein kinase ZAP-70 70 kDa zeta-chain associated protein Syk-related tyrosine kinase ZAP70 SRK |
| Molecular Weight(MW) | 70 kDa |
Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).
Application
Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Application
ZAP70 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
