Application
Western blot analysis of ZBRK1 in A-20 lysate with ZBRK1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | ZBRK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZBRK1 Antibody: ZFQR, ZBRK1, Zinc finger protein 350, KRAB zinc finger protein ZFQR |
| Product description | ZBRK1 Antibody: ZBRK1 is a member of the Kruppel-associated box-zinc finger protein (KRAB-ZFP) protein family of transcriptional repressors. ZBRK1 has been shown to not only be up-regulated in senescent fibroblasts but also interacts with BRCA1, a transcriptional regulator with tumor suppressor activity. ZBRK1 binds to a specific sequence within the GADD45 gene, supporting the assembly of a nuclear complex containing BRCA1 and repressing its transcription. Recent experiments demonstrate that ZBRK1 can homo-oligomerize in vitro and in vivo through a C-terminal transcriptional repression domain (CTRD), and this oligomerization facilitates the ZBRK1-directed transcriptional repression through ZBRK1 response elements. These results suggest that higher order ZBRK1 oligomers may assemble through both protein-DNA and CTRD-dependent protein-protein interactions.1) Zheng L, Pan H, Li S, et al. Sequence-specific transcription corepressor function for BRCA1 through a novel Zinc finger protein, ZBRK1. Mol. Cell2000; 6:757-68. |
| Immunogen | ZBRK1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human ZBRK1. The immunogen is located within amino acids 410 - 460 of ZBRK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ZBRK1 antibody can be used for detection of ZBRK1 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZNF350 |
|---|---|
| Alternative Names | ZBRK1 Antibody: ZFQR ZBRK1 Zinc finger protein 350 KRAB zinc finger protein ZFQR |
Application
Western blot analysis of ZBRK1 in A-20 lysate with ZBRK1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | ZBRK1 antibody can be used for detection of ZBRK1 by Western blot at 1 μg/mL.: |
| Form | Liquid |
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| Storage Instructions | ZBRK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZBRK1 Antibody is supplied in PBS containing 0.02% sodium azide. |
