Application
Western blot analysis of ZBTB3 in rat brain tissue lysate with ZBTB3 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of ZBTB3 in human brain tissue with ZBTB3 antibody at 20 μg/mL.Application
Immunohistochemistry of ZBTB3 in human brain tissue with ZBTB3 antibody at 2.5 μg/mL.| Product Name | ZBTB3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZBTB3 Antibody: Zinc finger and BTB domain-containing protein 3 |
| Product description | ZBTB3 Antibody: The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. While little is known about ZBTB3, the related protein ZBTB2 is thought to be phosphorylated in response to the DNA damage, probably by either ATM or ATR. Other ZBTB proteins, such as ZBTB4 and ZBTB38 bind methylated DNA and repress transcription, suggesting that ZBTB3 may also act as a transcription repressor.1) Strausberg RL, Feingold EA, Grouse LH, et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc. Natl. Acad. Sci. USA2002; 99:16899-903. |
| Immunogen | ZBTB3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ZBTB3. The immunogen is located within amino acids 510 - 560 of ZBTB3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ZBTB3 antibody can be used for detection of ZBTB3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZBTB3 |
|---|---|
| Alternative Names | ZBTB3 Antibody: Zinc finger and BTB domain-containing protein 3 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least three isoforms of ZBTB3 are known to exist. This antibody is predicted to not cross-react with other ZBTB protein family members. |
Application
Western blot analysis of ZBTB3 in rat brain tissue lysate with ZBTB3 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of ZBTB3 in human brain tissue with ZBTB3 antibody at 20 μg/mL.
Application
Immunohistochemistry of ZBTB3 in human brain tissue with ZBTB3 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | ZBTB3 antibody can be used for detection of ZBTB3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ZBTB3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZBTB3 Antibody is supplied in PBS containing 0.02% sodium azide. |
