ZBTB38 physically associates with CtBP via a conserved CtBP binding motif, PLDLR. When heterologously targeted to DNA, it represses transcription via two independent repression domains, an N-terminal BTB domain and a PLDLR motif-containing RD2 region, in a histone deacetylase-independent and -dependent manner, respectively1) Sasai, N., et al., (2005) Genes Cells 10 (9), 871-885.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse ZBTB38.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ZBTB38 antibody can be used for detection of ZBTB38 by ELISA at 1:312500. ZBTB38 antibody can be used for detection of ZBTB38 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Mouse SP2/0 at 0.2-1 ug/ml.
Positive Control
1) SP2/0 Cell Lysate
Application Notes
ZBTB38 antibody can be used for detection of ZBTB38 by ELISA at 1:312500. ZBTB38 antibody can be used for detection of ZBTB38 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ZBTB38 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.