Application
Western blot analysis of ZBTB6 in EL4 cell lysate with ZBTB6 antibody at 1 μg/mL.Application
Immunofluorescence of ZBTB6 in EL4 cells with ZBTB6 antibody at 20 μg/mL.Application
Immunocytochemistry of ZBTB6 in EL4 cells with ZBTB6 antibody at 5 μg/mL.| Product Name | ZBTB6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZBTB6 Antibody: ZID, ZNF482, ZID, Zinc finger and BTB domain-containing protein 6, Zinc finger protein 482 |
| Product description | ZBTB6 Antibody: The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB6, also known as ZNF482, is highly expressed in brain. While little is known about ZBTB6, other ZBTB proteins, such as ZBTB4 bind methylated DNA and repress transcription. Another ZBTB family member, ZBTB5, also acts as a transcriptional repressor, and can interact with co-repressor histone deacetylase complexes such as BCoR, NCoR, and SMRT via its POZ domain, suggesting that ZBTB6 may also act as a transcriptional repressor.1) Strausberg RL, Feingold EA, Grouse LH, et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc. Natl. Acad. Sci. USA2002; 99:16899-903. |
| Immunogen | ZBTB6 antibody was raised against a 15 amino acid synthetic peptide near the center of human ZBTB6. The immunogen is located within amino acids 180 - 230 of ZBTB6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| ZBTB6 antibody can be used for detection of ZBTB6 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZBTB6 |
|---|---|
| Alternative Names | ZBTB6 Antibody: ZID ZNF482 ZID Zinc finger and BTB domain-containing protein 6 Zinc finger protein 482 |
| Sequence Similarities | 2) Cat. No. 17-207 - EL4 Cell Slide |
| Tissue Specificity | At least two isoforms of ZBTB6 are known to exist; this antibody will only recognize the shorter isoform. This antibody is predicted to not cross-react with other ZBTB protein family members. |
Application
Western blot analysis of ZBTB6 in EL4 cell lysate with ZBTB6 antibody at 1 μg/mL.
Application
Immunofluorescence of ZBTB6 in EL4 cells with ZBTB6 antibody at 20 μg/mL.
Application
Immunocytochemistry of ZBTB6 in EL4 cells with ZBTB6 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | ZBTB6 antibody can be used for detection of ZBTB6 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ZBTB6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZBTB6 Antibody is supplied in PBS containing 0.02% sodium azide. |
