Application
Western blot analysis of ZBTB9 in mouse heart tissue lysate with ZBTB9 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of ZBTB9 in mouse heart tissue with ZBTB9 antibody at 20 μg/mL.Application
Immunohistochemistry of ZBTB9 in mouse heart tissue with ZBTB9 antibody at 2.5 μg/mL.| Product Name | ZBTB9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZBTB9 Antibody: ZNF919, Zinc finger and BTB domain-containing protein 9 |
| Product description | ZBTB9 Antibody: The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB9, also known as ZNF919, is present in multiple tissues, and is highly expressed in the cytoplasm of the placenta. While little is known about ZBTB9, other ZBTB proteins, such as ZBTB4 bind methylated DNA and repress transcription. Another ZBTB proteins, ZBTB7A, has been implicated as a proto-oncogene whose overexpression contributes to malignancy in breast cancer, suggesting that ZBTB9 may act as a transcriptional repressor or be involved in tumorigenesis.1) Filion GJP, Zhenilo S, Salozhin S, et al. A family of zinc finger proteins that bind methylated DNA and repress transcription. Mol. Cell. Biol. 2006; 26:169-81. |
| Immunogen | ZBTB9 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ZBTB9. The immunogen is located within the first 50 amino acids of ZBTB9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| ZBTB9 antibody can be used for detection of ZBTB9 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZBTB9 |
|---|---|
| Alternative Names | ZBTB9 Antibody: ZNF919 Zinc finger and BTB domain-containing protein 9 |
| Tissue Specificity | This antibody is predicted to not cross-react with other ZBTB protein family members. |
Application
Western blot analysis of ZBTB9 in mouse heart tissue lysate with ZBTB9 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of ZBTB9 in mouse heart tissue with ZBTB9 antibody at 20 μg/mL.
Application
Immunohistochemistry of ZBTB9 in mouse heart tissue with ZBTB9 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1401 - Mouse Heart Tissue Lysate |
|---|---|
| Application Notes | ZBTB9 antibody can be used for detection of ZBTB9 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ZBTB9 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZBTB9 Antibody is supplied in PBS containing 0.02% sodium azide. |
