Zinc finger proteins (Zfp) are encoded by a large family of genes present in many organisms including yeast and human. Some of them are transcriptional activators and bind specifically to DNA by zinc mediated folded structures commonly known as zinc fingers. The putative Zfp-1 (Zinc finger protein 1 homolog) protein contains in addition to 7 zinc fingers, two helix-turn-helix motifs.1) Chowdhury, K., et al., (1989) NucleicAcidsRes.17(24), 10427-10438.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZFP1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
ZFP1 antibody can be used for detection of ZFP1 by ELISA at 1:62500. ZFP1 antibody can be used for detection of ZFP1 by western blot at 0.1-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in IHC on Human Spermatophore at 4.0-8.0 ug/ml.
Application
Antibody used in IHC on Human Intestine at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
ZFP1 antibody can be used for detection of ZFP1 by ELISA at 1:62500. ZFP1 antibody can be used for detection of ZFP1 by western blot at 0.1-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ZFP1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.