Application
Western blot analysis of ZFX in Daudi cell lysate with ZFX antibody at 1 μg/mL.Application
Immunofluorescence of ZFX in human small intestine tissue with ZFX antibody at 20 μg/mL.Application
Immunohistochemistry of ZFX in human small intestine tissue with ZFX antibody at 5 μg/mL.| Product Name | ZFX Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ZFX Antibody: The X-linked zinc finger protein ZFX is a member of the Kruppel C2H2-type zinc-finger protein family. The full-length protein contains an acidic transcriptional activation domain (AD), a nuclear localization sequence (NLS) and a DNA binding domain (DBD) consisting of 13 C2H2-type zinc fingers. Studies in mouse embryonic and adult hematopoietic stem cells showed that this gene was required as a transcriptional regulator for self-renewal of both stem cell types, but it was dispensable for growth and differentiation of their progeny.1) Schneider-Gadicke A, Beer-Romero P, Brown LG, et al. Putative transcription activator with alternative isoforms encoded by human ZFX gene. Nature 1989; 342:708-11. |
| Immunogen | ZFX antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ZFX. The immunogen is located within the last 50 amino acids of ZFX. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ZFX antibody can be used for detection of ZFX by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZFX |
|---|---|
| Alternative Names | ZFX Antibody: ZNF926 Zinc finger X-chromosomal protein |
| Molecular Weight(MW) | Predicted: 89, 63 kDa Observed: 63 kDa |
| Sequence Similarities | 3) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
| Tissue Specificity | ZFX antibody is human, mouse and rat reactive. Multiple isoforms of ZFX are known to exist. ZFX antibody will detect isoform 1 and 2 of ZFX. |
Application
Western blot analysis of ZFX in Daudi cell lysate with ZFX antibody at 1 μg/mL.
Application
Immunofluorescence of ZFX in human small intestine tissue with ZFX antibody at 20 μg/mL.
Application
Immunohistochemistry of ZFX in human small intestine tissue with ZFX antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | ZFX antibody can be used for detection of ZFX by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | ZFX antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZFX Antibody is supplied in PBS containing 0.02% sodium azide. |
