Application
Western blot analysis of ZIP1 in mouse kidney tissue lysate with ZIP1 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of ZIP1 in human kidney tissue with ZIP1 antibody at 20 μg/mL.Application
Immunohistochemistry of ZIP1 in human kidney tissue with ZIP1 antibody at 2.5 μg/mL.| Product Name | ZIP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ZIP1 Antibody: ZIP1, also known as ZIRTL (zinc-iron regulated transporter-like), is the first mammalian member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP1 expression is markedly downregulated in a number of cancerous tissues and is thought to function as a tumor suppressor gene in prostate cancer. More recent studies have shown that overexpression of ZIP1 and concomitant increased levels of intracellular zinc in PC-3 cells cause a significant inhibition of NF-κB, leading to down-regulation of the antiapoptotic protein Bcl-2, Bcl-XL, and the apoptosis inhibitor XIAP, reducing the malignant potential of prostate cancer cells in vitro and in vivo.1) Lioumi M, Ferguson CA, Sharpe PT, et al. Isolation and characterization of human and mouse ZIRTL, a member of the IRT1 family of transporters, mapping within the epidermal differentiation complex. Genomics 1999; 62:272-80. |
| Immunogen | ZIP1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human ZIP1. The immunogen is located within amino acids 40 - 90 of ZIP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ZIP1 antibody can be used for detection of ZIP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC39A1 |
|---|---|
| Alternative Names | ZIP1 Antibody: ZIP1 ZIRTL IRT1 ZIP1 CGI-08 CGI-71 Zinc transporter ZIP1 Solute carrier family 39 member 1 ZIP-1 |
| Molecular Weight(MW) | Predicted: 13, 36 kDa Observed: 38 kDa |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | At least two isoforms of ZIP1 are known to exist; this antibody will detect both isoforms. ZIP1 antibody is predicted not to cross-react with other ZIP family proteins. |
Application
Western blot analysis of ZIP1 in mouse kidney tissue lysate with ZIP1 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of ZIP1 in human kidney tissue with ZIP1 antibody at 20 μg/mL.
Application
Immunohistochemistry of ZIP1 in human kidney tissue with ZIP1 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate |
|---|---|
| Application Notes | ZIP1 antibody can be used for detection of ZIP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | ZIP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZIP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
