Application
Western blot analysis of ZIP5 in human spleen tissue lysate with ZIP5 antibody at 1 μg/mL.Application
Immunofluorescence of ZIP5 in human spleen tissue with ZIP5 antibody at 20 μg/mL.Application
Immunohistochemistry of ZIP5 in human spleen tissue with ZIP5 antibody at 2.5 μg/mL.| Product Name | ZIP5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ZIP5 Antibody: The zinc transporter ZIP5, also known as SLC39A5, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP5 is closely related to ZIP4, and is found in many of the same tissues, including intestine, pancreas, liver, and kidney, but unlike ZIP4, ZIP5 localizes to the basolateral membrane in kidney cells and its activity is not downregulated by zinc repletion. Recent evidence suggests that ZIP4 and ZIP5 are reciprocally regulated, with translation of ZIP5 mRNA decreasing and ZIP5 protein being rapidly internalized and degraded in response to zinc deficiency.1) Dufner-Beattie J, Langmade SJ, Wang F, et al. Structure, function, and regulation of a subfamily of mouse zinc transporter genes. J. Biol. Chem.2003; 278:50142-50. |
| Immunogen | ZIP5 antibody was raised against a 17 amino acid synthetic peptide near the center of human ZIP5. The immunogen is located within amino acids 330 - 380 of ZIP5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ZIP5 antibody can be used for detection of ZIP5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC39A5 |
|---|---|
| Alternative Names | ZIP5 Antibody: ZIP5 LZT-Hs7 ZIP5 Zinc transporter ZIP5 Solute carrier family 39 member 5 ZIP-5 |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Entrez Gene | 283375 |
|---|---|
| Protein Accession | NP_001128667 |
Application
Western blot analysis of ZIP5 in human spleen tissue lysate with ZIP5 antibody at 1 μg/mL.
Application
Immunofluorescence of ZIP5 in human spleen tissue with ZIP5 antibody at 20 μg/mL.
Application
Immunohistochemistry of ZIP5 in human spleen tissue with ZIP5 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | ZIP5 antibody can be used for detection of ZIP5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | ZIP5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZIP5 Antibody is supplied in PBS containing 0.02% sodium azide. |
