Application
Western blot analysis of ZIP kinase in (A) HeLa and (B) Jurkat lysates with ZIP kinase antibody at 1 μg/mL.Application
Immunofluorescence of ZIPK in Jurkat cells with ZIPK antibody at 10 μg/mL.Application
Immunocytochemistry of ZIPK in Jurkat cells with ZIPK antibody at 5 μg/ml.Application
Immunofluorescence of ZIPK in Jurkat cells with ZIPK antibody at 5 μg/ml.Application
Immunocytochemistry of ZIP kinase in Jurkat cells with ZIP kinase antibody at 10 μg/mL.| Product Name | ZIPK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ZIPK Antibody: Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase. ZIP kinase contains an N-terminal kinase domain and a C-terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREB family. ZIP kinase has high sequence homology to DAP kinase (death-associated protein kinase), which is a mediator of apoptosis induced by gamma interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues.1) Kawai T, Matsumoto M, Takeda K, Sanjo H, Akira S. ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. Mol Cell Biol 1998;18:1642-51 (RD1299) |
| Immunogen | ZIP kinase antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase. The immunogen is located within amino acids 270 - 320 of ZIPK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| ZIPK antibody can be used for detection of DNase II expression by Western blot at 1 μg/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DAPK3 |
|---|---|
| Alternative Names | ZIPK Antibody: ZIP ZIPK Death-associated protein kinase 3 DAP-like kinase DAP kinase 3 |
| Molecular Weight(MW) | Predicted: 26 kDa Observed: 27 kDa |
| Sequence Similarities | 4) Cat. No. 1208 - THP-1 Cell Lysate |
Application
Western blot analysis of ZIP kinase in (A) HeLa and (B) Jurkat lysates with ZIP kinase antibody at 1 μg/mL.
Application
Immunofluorescence of ZIPK in Jurkat cells with ZIPK antibody at 10 μg/mL.
Application
Immunocytochemistry of ZIPK in Jurkat cells with ZIPK antibody at 5 μg/ml.
Application
Immunofluorescence of ZIPK in Jurkat cells with ZIPK antibody at 5 μg/ml.
Application
Immunocytochemistry of ZIP kinase in Jurkat cells with ZIP kinase antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | ZIPK antibody can be used for detection of DNase II expression by Western blot at 1 μg/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | ZIPK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZIPK Antibody is supplied in PBS containing 0.02% sodium azide. |
