Application
Western blot analysis in U251 cell line lysates (35ug/lane).Application
ZNF197 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | ZNF197 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Zinc finger protein 197, Zinc finger protein with KRAB and SCAN domains 9, ZnF20, pVHL-associated KRAB domain-containing protein, ZNF197, ZKSCAN9, ZNF166 |
| Product description | ZNF197 product belongs to the zinc finger protein superfamily, members of which are regulatory proteins characterized by nucleic acid-binding zinc finger domains. The encoded protein contains 20 tandemly arrayed C2H2-type zinc fingers, a Kruppel-associated box (KRAB) domain, and a SCAN box. This transcript turns over rapidly and contains 3' UTR AUUUA motifs, which are often a hallmark of rapid turnover. It is overexpressed in some thyroid papillary carcinomas. ZNF197 is located in a cluster of zinc finger genes at 3p21. Two alternatively spliced transcripts encoding different isoforms have been described.1) Li, Z., et al. EMBO J. 22(8):1857-1867(2003) |
| Immunogen | This ZNF197 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314-343 amino acids from the N-terminal region of human ZNF197. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZNF197 |
|---|---|
| Alternative Names | Zinc finger protein 197 Zinc finger protein with KRAB and SCAN domains 9 ZnF20 pVHL-associated KRAB domain-containing protein ZNF197 ZKSCAN9 ZNF166 |
| Molecular Weight(MW) | 119 kDa |
Application
Western blot analysis in U251 cell line lysates (35ug/lane).
Application
ZNF197 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
