Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Product Name | ZNF265 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZNF265, ZIS, ZIS1, ZIS2, ZNF265 |
| Product description | ZNF265 is a protein that has been shown to bind to the spliceosomal components U1-70K and U2AF35 and to direct alternative splicing. Analysis of the structure reveals substantial similarity to known RNA-binding motifs in terms of the distribution of key surface residues responsible for making RNA contacts, despite a complete lack of structural homology. An RNA gel shift assay was used to demonstrate that a single crossed finger domain from ZNF265 is capable of binding to an RNA message. Taken together, these results define a new RNA-binding motif and should provide insight into the functions of the >100 uncharacterized proteins in the sequence data bases that contain this domain.1) Plambeck, C.A., et al., (2003) J. Biol. Chem. 278 (25), 22805-22811. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZNF265. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ZNF265 antibody can be used for detection of ZNF265 by ELISA at 1:312500. ZNF265 antibody can be used for detection of ZNF265 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZRANB2 |
|---|---|
| Alternative Names | ZNF265 ZIS ZIS1 ZIS2 ZNF265 |
| Molecular Weight(MW) | 37 kDa, 36 kDa |
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | ZNF265 antibody can be used for detection of ZNF265 by ELISA at 1:312500. ZNF265 antibody can be used for detection of ZNF265 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ZNF265 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
