Application
Antibody used in IHC on Human Stomach.Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | ZNF322A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZNF322A, HCG12, ZNF388, ZNF489, ZNF322A |
| Product description | ZNF322 contains four exons and spans 23.2kb in chromosome 6p22.1 region, and transcribes a 2.7kb mRNA that encodes a protein with 402 amino acid residues.Through northern blot analysis, ZNF322 was shown to be expressed in every human tissue examined at adult stage and during embryonic developmental stages from 80 days to 24 weeks. When ZNF322 was overexpressed in COS-7 cells, ZNF322-EGFP fusion protein is detected in the nucleus and cytoplasm. Reporter gene assays show that ZNF322 is a transcriptional activator. Furthermore, overexpression of ZNF322 in COS-7 cells activates the transcriptional activity of SRE and AP-1. Together, these results suggest that ZNF322 is a member of the zinc-finger transcription factor family and may act as a positive regulator in gene transcription mediated by the MAPK signaling pathways.1) Li, Y., (2004) Biochem. Biophys. Res. Commun. 325 (4), 1383-1392. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZNF322A. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| ZNF322A antibody can be used for detection of ZNF322A by ELISA at 1:12500. ZNF322A antibody can be used for detection of ZNF322A by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
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| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZNF322 |
|---|---|
| Alternative Names | ZNF322A HCG12 ZNF388 ZNF489 ZNF322A |
| Molecular Weight(MW) | 47 kDa, 47 kDa |
Application
Antibody used in IHC on Human Stomach.
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | ZNF322A antibody can be used for detection of ZNF322A by ELISA at 1:12500. ZNF322A antibody can be used for detection of ZNF322A by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ZNF322A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
