ZNF449 belongs to the krueppel C2H2-type zinc-finger protein family. It contains 7 C2H2-type zinc fingers and 1 SCAN box domain. ZNF449 may be involved in transcriptional regulation.1) Luo, K., (2006) Mol. Biol. Rep. 33 (1), 51-57.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZNF449.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ZNF449 antibody can be used for detection of ZNF449 by ELISA at 1:312500. ZNF449 antibody can be used for detection of ZNF449 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Transfected 293T cell lysates at 0.2-1 ug/ml.
Positive Control
1) Tranfected 293T Cell Lysate
Application Notes
ZNF449 antibody can be used for detection of ZNF449 by ELISA at 1:312500. ZNF449 antibody can be used for detection of ZNF449 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ZNF449 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.