ZNF540 belongs to the krueppel C2H2-type zinc-finger protein family. It contains 17 C2H2-type zinc fingers and 1 KRAB domain. ZNF540 may be involved in transcriptional regulation.1) Xiang, Z., (2006) Biochem. Biophys. Res. Commun. 347 (1), 288-296.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZNF540.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ZNF540 antibody can be used for detection of ZNF540 by ELISA at 1:62500. ZNF540 antibody can be used for detection of ZNF540 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1219 - MCF7 Cell Lysate
Application Notes
ZNF540 antibody can be used for detection of ZNF540 by ELISA at 1:62500. ZNF540 antibody can be used for detection of ZNF540 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ZNF540 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.