Application
Immunohistochemical analysis of paraffin-embedded H. prostate section using ZRSR2 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.Application
Western blot analysis in 293,HepG2,HL-60,Jurkat,MCF-7,ZR-75-1 cell line lysates (35ug/lane).| Product Name | ZRSR2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2, CCCH type zinc finger, RNA-binding motif and serine/arginine rich protein 2, Renal carcinoma antigen NY-REN-20, U2(RNU2) small nuclear RNA auxiliary factor 1-like 2, U2AF |
| Product description | Pre-mRNA-binding protein required for splicing of both U2-and U12-type introns. Selectively interacts with the 3'-splice site of U2-and U12-type pre-mRNAs and promotes different steps in U2 and U12 intron splicing. Recruited to U12 pre-mRNAs in an ATP-dependent manner and is required for assembly of the prespliceosome, a precursor to other spliceosomal complexes. For U2-type introns, it is selectively and specifically required for the second step of splicing.1) Kitagawa K., et al. Genomics 30:257-263(1995). |
| Immunogen | This ZRSR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 453-482 amino acids from the C-terminal region of human ZRSR2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZRSR2 |
|---|---|
| Alternative Names | U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2 CCCH type zinc finger RNA-binding motif and serine/arginine rich protein 2 Renal carcinoma antigen NY-REN-20 U2(RNU2) small nuclear RNA auxiliary factor 1-like 2 U2AF |
| Molecular Weight(MW) | 58 kDa |
Application
Immunohistochemical analysis of paraffin-embedded H. prostate section using ZRSR2 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Application
Western blot analysis in 293,HepG2,HL-60,Jurkat,MCF-7,ZR-75-1 cell line lysates (35ug/lane).| Application Notes | For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
