Application
Western blot analysis of ZSCAN4 in mouse lung tissue lysate with ZSCAN4 antibody in (A) the absence and (B) presence of blocking peptide.Application
Immunofluorescence of ZSCAN4 in Rat Lung tissue with ZSCAN4 antibody at 20 μg/mL.Application
Immunohistochemistry of ZSCAN4 in rat lung tissue with ZSCAN4 antibody at 5 μg/mL.| Product Name | ZSCAN4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ZSCAN4 Antibody: Zytotic genome activation (ZGA) is the first and most critical transition event from the maternal genetic to the embryonic genetic program and the major burst of ZGA occurs only in the late 2-cell stage. ZSCAN4 encodes a SCAN domain and four zinc finger domains and is expressed in late 2-cell embryos and embryonic stem cells. Loss-of-function studies using siRNA shows delays in the progression from the 2-cell to the 4-cell stage and subsequent embryo development. ZSCAN4 plays an important role in the preimplantation development. Also, ZSCAN4 is involved in telomere maintenance and long term genomic stability in ES cells, suggesting that ZSCAN4 may be of critical importance in maintaining pluripotency in replicating stem cells.1) Hamatani T, Carter MG, Sharov AA, et al. Dynamics of global gene expression changes during mouse preimplantation development. Dev. Cell 2004; 6:117-131. |
| Immunogen | ZSCAN4 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ZSCAN4. The immunogen is located within amino acids 370 - 420 of ZSCAN4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ZSCAN4 antibody can be used for detection of ZSCAN4 by Western blot. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZSCAN4 |
|---|---|
| Alternative Names | ZSCAN4 Antibody: ZNF494 ZNF494 Zinc finger and SCAN domain-containing protein 4 Zinc finger protein 494 |
| Molecular Weight(MW) | Predicted: 48 kDa Observed: 64 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (81%), Chicken: (86%) |
| Entrez Gene | 201516 |
|---|---|
| Protein Accession | NP_689890.1 |
Application
Western blot analysis of ZSCAN4 in mouse lung tissue lysate with ZSCAN4 antibody in (A) the absence and (B) presence of blocking peptide.
Application
Immunofluorescence of ZSCAN4 in Rat Lung tissue with ZSCAN4 antibody at 20 μg/mL.
Application
Immunohistochemistry of ZSCAN4 in rat lung tissue with ZSCAN4 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1402 - Mouse Lung Tissue Lysate |
|---|---|
| Application Notes | ZSCAN4 antibody can be used for detection of ZSCAN4 by Western blot. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ZSCAN4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZSCAN4 Antibody is supplied in PBS containing 0.02% sodium azide. |
