Application
Immunofluorescent analysis of HeLa cells using β-Catenin (L54E2) Mouse mAb (IF Preferred) detected with Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate) (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).Application
Flow cytometric analysis of untreated Jurkat cells using Akt (5G3) Mouse mAb detected with Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate) (blue) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control (red).| Product Name | Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate) |
|---|---|
| Antibody Type | Secondary Antibodies |
| Product description | Anti-mouse IgG (H+L), F(ab');2; Fragment was conjugated to Alexa Fluor;® ;594 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab');2; fragment results in less non-specific binding to cells through Fc receptors. |
| Clonality | Monoclonal |
|---|---|
| Tested Applications | WBIPIHCIFFCChIP |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Tissue Specificity | F(ab');2; fragments are prepared from goat antibodies that have been adsorbed against human IgG and human serum. |
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Application
Immunofluorescent analysis of HeLa cells using β-Catenin (L54E2) Mouse mAb (IF Preferred) detected with Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate) (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).
Application
Flow cytometric analysis of untreated Jurkat cells using Akt (5G3) Mouse mAb detected with Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate) (blue) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control (red).| Storage Instructions | The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays.; ;Storage: ; Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. ;Do not aliquot the antibody. Protect from light. Do not freeze. |
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| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
