Application
Confocal immunofluorescent analysis of HeLa cells labeled with MEK1/2 (47E6) Rabbit mAb detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate) (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin (green). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).Application
Confocal immunofluorescent analysis of mouse cerebellum using α-Synuclein Antibody (IF Preferred) detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate) (red) and Neurofilament-L (DA2) Mouse mAb detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) (green). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).| Product Name | Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate) |
|---|---|
| Antibody Type | Secondary Antibodies |
| Product description | Anti-Rabbit IgG (H+L) F(ab');2; Fragment was conjugated to Alexa Fluor;®; 555 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab');2; fragment results in less non-specific binding to cells through Fc receptors. |
| Clonality | Monoclonal |
|---|---|
| Tested Applications | WBIPIHCIFFCChIP |
| Species Reactivity | Rabbit |
| Concentration | 1mg/ml |
| Tissue Specificity | F(ab');2; fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins and purified human paraproteins. |
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Application
Confocal immunofluorescent analysis of HeLa cells labeled with MEK1/2 (47E6) Rabbit mAb detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate) (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin (green). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).
Application
Confocal immunofluorescent analysis of mouse cerebellum using α-Synuclein Antibody (IF Preferred) detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate) (red) and Neurofilament-L (DA2) Mouse mAb detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) (green). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).| Storage Instructions | The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:1000 should yield acceptable results for immunofluorescent assays.; ;Storage: ; Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. ;Do not aliquot the antibody. Protect from light. Do not freeze. |
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| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
