Application
Confocal immunofluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb detected with Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate) (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).Application
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 (blue), using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb detected with Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate).| Product Name | Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate) |
|---|---|
| Antibody Type | Secondary Antibodies |
| Product description | Anti-rabbit IgG (H+L), F(ab')2 Fragment was conjugated to Alexa Fluor® 594 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Alligator |
| Tested Applications | ChIPFCIFIHCIPWB |
| The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution shown below should yield acceptable results for immunofluorescent and flow cytometry assays:Immunofluorescent: 1:1000Flow Cytometry: 1:500: |
|
| Species Reactivity | Rabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Tissue Specificity | F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins, and purified human paraproteins. |
|---|
Application
Confocal immunofluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb detected with Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate) (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).
Application
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 (blue), using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb detected with Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 594 Conjugate).| Application Notes | The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution shown below should yield acceptable results for immunofluorescent and flow cytometry assays:Immunofluorescent: 1:1000Flow Cytometry: 1:500: |
|---|
| Storage Instructions | Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. |
|---|---|
| Storage Buffer | Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. |
