Application
Western blot analysis of Jurkat cell lysates (#9194) treated with either U0126 (MEK 1/2 inhibitor) or TPA (12-O-Tetradecanoylphorbol-13-Acetate) using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb detected with Anti-rabbit IgG (H+L) (DyLight™ 680 Conjugate) (red) and Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb detected with Anti-mouse IgG (H+L) (DyLight™ 800 Conjugate) (green). The array image pixel intensities obtained using a LI-COR ® Biosciences Odyssey ® Infrared Imaging System are shown in the upper panel while corresponding fluorescent western blots are shown in the lower panel.Application
In-Cell Western™ analysis of A549 cells exposed to varying concentrations of U0126 (MEK1/2 Inhibitor) for 3 hours, followed by TPA (Phorbol-12-Myristate-13-Acetate) stimulation for 30 minutes. With increasing concentrations of U0126, a significant decrease (~5 fold) in Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb signal as compared to the TPA-stimulated control was observed. When using phospho-Erk as a measurement, the IC 50 of this compound was 1.9 μM. Data and images were generated on the LI-COR ® Biosciences Odyssey ® Infrared Imaging System using Anti-rabbit IgG (H+L) (DyLight™ 680 Conjugate).| Product Name | Anti-rabbit IgG (H+L) (DyLight™ 680 Conjugate) |
|---|---|
| Antibody Type | Secondary Antibodies |
| Product description | Anti-rabbit IgG (H+L) was conjugated to DyLight™ 680 fluorescent dye under optimal conditions and formulated at 1 mg/ml. Excitation is 684 nm and peak fluorescence emission is 715 nm. |
| Clonality | Monoclonal |
|---|---|
| Tested Applications | WB |
| Species Reactivity | Rabbit |
| Concentration | 1mg/ml |
| Tissue Specificity | Anti-rabbit IgG (H+L) (DyLight™ 680 Conjugate) reacts with heavy and light chain of most rabbit immunoglobulins. No cross-reactivity to other serum proteins has been detected. This antibody may cross-react with immunoglobulins from other species. |
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Application
Western blot analysis of Jurkat cell lysates (#9194) treated with either U0126 (MEK 1/2 inhibitor) or TPA (12-O-Tetradecanoylphorbol-13-Acetate) using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb detected with Anti-rabbit IgG (H+L) (DyLight™ 680 Conjugate) (red) and Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb detected with Anti-mouse IgG (H+L) (DyLight™ 800 Conjugate) (green). The array image pixel intensities obtained using a LI-COR ® Biosciences Odyssey ® Infrared Imaging System are shown in the upper panel while corresponding fluorescent western blots are shown in the lower panel.
Application
In-Cell Western™ analysis of A549 cells exposed to varying concentrations of U0126 (MEK1/2 Inhibitor) for 3 hours, followed by TPA (Phorbol-12-Myristate-13-Acetate) stimulation for 30 minutes. With increasing concentrations of U0126, a significant decrease (~5 fold) in Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb signal as compared to the TPA-stimulated control was observed. When using phospho-Erk as a measurement, the IC 50 of this compound was 1.9 μM. Data and images were generated on the LI-COR ® Biosciences Odyssey ® Infrared Imaging System using Anti-rabbit IgG (H+L) (DyLight™ 680 Conjugate).| Storage Instructions | The optimal dilution of the anti-species antibody should be determined by the user. However, the final dilutions below should yield acceptable results for the respective applications.;Fluorescent western blotting: 1:15000;In-Cell Western: 1:500; ;Storage: ; Supplied in 100 mM PBS, pH 7.2, containing 1% BSA and 0.02% sodium azide. Store at 4°C. Protect from light. ;Do not freeze. |
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| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
