Application
Immunoprecipitation of Jurkat cell extracts using Akt (pan) (C67E7) Rabbit mAb #4691. Extracts were incubated with either Anti-Rabbit IgG F(ab') 2 Fragment (Sepharose ® Bead Conjugate) (lane 1) or Protein A Beads (lane 2). The western blot was probed using Akt (pan) (40D4) Mouse mAb #2920.| Product Name | Anti-rabbit IgG F(ab') 2 Fragment (Sepharose ® Bead Conjugate) |
|---|---|
| Antibody Type | Secondary Antibodies |
| Product description | This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose;®; beads. Anti-rabbit IgG F(ab') ;2; Fragment (Sepharose;®; Bead Conjugate) is useful for the immunoprecipitation of antibodies raised in rabbit. |
| Clonality | Monoclonal |
|---|---|
| Tested Applications | WBIPIHCIFFCChIP |
| Species Reactivity | Rabbit |
| Concentration | 1mg/ml |
| Tissue Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Rabbit IgG and Rabbit serum. No reaction was observed against anti-pepsin, anti-goat IgG F;c;, or human or mouse serum proteins. |
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Application
Immunoprecipitation of Jurkat cell extracts using Akt (pan) (C67E7) Rabbit mAb #4691. Extracts were incubated with either Anti-Rabbit IgG F(ab') 2 Fragment (Sepharose ® Bead Conjugate) (lane 1) or Protein A Beads (lane 2). The western blot was probed using Akt (pan) (40D4) Mouse mAb #2920.| Storage Instructions | Add 10-20 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details.; ;Storage: ; Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies. |
|---|---|
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
