Application
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 (blue), using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb #4060. Anti-Rabbit IgG (H+L), (PE Conjugate) was used as a secondary antibody.| Product Name | Anti-Rabbit IgG (H+L), (PE Conjugate) |
|---|---|
| Antibody Type | Secondary Antibodies |
| Product description | Affinity purified goat anti-rabbit IgG (H+L) antibody is conjugated to R-phycoerythrin. This product has been optimized for use as a secondary antibody in flow applications. Excitation is 450-580 nm and peak emission is 590 nm. |
| Clonality | Monoclonal |
|---|---|
| Tested Applications | WBIPIHCIFFCChIP |
| Species Reactivity | Rabbit |
| Concentration | 1mg/ml |
| Tissue Specificity | Anti-Rabbit IgG (H+L), (PE Conjugate) reacts with heavy and light chain of most rabbit immunoglobulins. No cross-reactivity to other serum proteins has been detected.;Less than 1% cross-reactivity is observed with immunoglobulins from other species. |
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Application
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 (blue), using Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb #4060. Anti-Rabbit IgG (H+L), (PE Conjugate) was used as a secondary antibody.| Storage Instructions | The optimal dilution of the secondary antibody should be determined for each application. However, a final dilution of 1:500 should yield acceptable results for most flow cytometry applications.; ;Storage: ; Supplied in 10 mM HEPES, 150 mM NaCl, pH 7.4, 0.08% sodium azide. Store at 4°C. Protect from light for up to one year. ;Do not freeze. |
|---|---|
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
