Application
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO ® is added and emits light during enzyme catalyzed decomposition.WB
(B) Western blot analysis of MEIS1, LIF and HOXA10 protein levels in the endometrium from women undergoing three different COS protocols, and in an unstimulated control group. The marker protein levels were calculated as a ratio of their densitometric value to that of the GAPDH reference gene, and are represented in the right panel. N = 3 for western blot analysis. The densitometric values for the GAPDH reference protein were comparable for all samples. For comparison between treatment groups, protein expression level ratios were defined as 1 in the control groups. #The difference in mRNA and protein expression between the COS groups and the unstimulated control group was statistically significant for HOXA10 and MEIS1 genes (P < 0.05, one-way ANOVA with Bonferroni post hoc test).| Product Name | Anti-rabbit IgG, HRP-linked Antibody |
|---|---|
| Antibody Type | Secondary Antibodies |
| Product description | Designed for use with rabbit polyclonal and monoclonal antibodies, this affinity purified goat anti-rabbit IgG (heavy and light chain) antibody is conjugated to horseradish peroxidase(HRP) for chemiluminescent detection. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Goat |
| Tested Applications | ChIPFCIFIHCIPWB |
| WB:1:5000~1:10000 |
|
| Species Reactivity | Rabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Application
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO ® is added and emits light during enzyme catalyzed decomposition.
WB
(B) Western blot analysis of MEIS1, LIF and HOXA10 protein levels in the endometrium from women undergoing three different COS protocols, and in an unstimulated control group. The marker protein levels were calculated as a ratio of their densitometric value to that of the GAPDH reference gene, and are represented in the right panel. N = 3 for western blot analysis. The densitometric values for the GAPDH reference protein were comparable for all samples. For comparison between treatment groups, protein expression level ratios were defined as 1 in the control groups. #The difference in mRNA and protein expression between the COS groups and the unstimulated control group was statistically significant for HOXA10 and MEIS1 genes (P < 0.05, one-way ANOVA with Bonferroni post hoc test).| Application Notes | WB:1:5000~1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
