Application
Western blot analysis of extracts from HeLa cells using β-Actin (8H10D10) Mouse mAb as the primary antibody. Rabbit Anti-mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) (left) and Rabbit Anti-mouse IgG (HRP Conjugate) (right) secondary antibodies were used.Application
Western blot analysis of decreasing concentrations of total mouse IgG reduced and denatured in 1x SDS loading buffer with DTT using Rabbit Anti-Mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) (left) or Anti-Mouse IgG (HRP Conjugate) (right).| Product Name | Rabbit Anti-Mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) |
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| Antibody Type | Secondary Antibodies |
| Product description | This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. |
| Clonality | Monoclonal |
|---|---|
| Tested Applications | WBIPIHCIFFCChIP |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Tissue Specificity | Rabbit Anti-mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) recognizes native mouse IgG. It also recognizes the denatured and reduced mouse IgG light chains (about 25 kDa) on a western blot. |
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Application
Western blot analysis of extracts from HeLa cells using β-Actin (8H10D10) Mouse mAb as the primary antibody. Rabbit Anti-mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) (left) and Rabbit Anti-mouse IgG (HRP Conjugate) (right) secondary antibodies were used.
Application
Western blot analysis of decreasing concentrations of total mouse IgG reduced and denatured in 1x SDS loading buffer with DTT using Rabbit Anti-Mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) (left) or Anti-Mouse IgG (HRP Conjugate) (right).| Storage Instructions | ; ; ;Application; ;Dilutions; ; ; ; ; ;Western Blotting; ;1:1000; ; ; ; ;Storage: ; Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
|---|---|
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
