Application
Western blot analysis of decreasing concentrations of total rabbit IgG reduced and denatured in 1X SDS loading buffer with DTT using Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (Panel A), Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb (Panel B), or Anti-rabbit IgG, HRP-linked Antibody (Panel C). For Panels A and B, the bound mouse anti-rabbit IgG mAb was detected by Anti-mouse IgG, HRP-linked Antibody #7076. The positions of the reduced and denatured rabbit IgG heavy and light chains are indicated.| Product Name | Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb |
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| Antibody Type | Secondary Antibodies |
| Product description | Affinity purified mouse anti-rabbit IgG (Light-Chain Specific) antibody. This product has been optimized for use as a secondary antibody in Western blotting applications. |
| Clonality | Monoclonal |
|---|---|
| Tested Applications | WBIPIHCIFFCChIP |
| Species Reactivity | Rabbit |
| Concentration | 1mg/ml |
| Tissue Specificity | Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb recognizes the light chain of rabbit IgG, either in native conformation or denatured and reduced form. It does not bind to the denatured and reduced rabbit IgG heavy chain (about 50 kDa) on western blot. |
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Application
Western blot analysis of decreasing concentrations of total rabbit IgG reduced and denatured in 1X SDS loading buffer with DTT using Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (Panel A), Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb (Panel B), or Anti-rabbit IgG, HRP-linked Antibody (Panel C). For Panels A and B, the bound mouse anti-rabbit IgG mAb was detected by Anti-mouse IgG, HRP-linked Antibody #7076. The positions of the reduced and denatured rabbit IgG heavy and light chains are indicated.| Storage Instructions | Storage: ; Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
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| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
