Application
Immunohistochemical analysis of paraffin-embedded human seminoma using Nanog (1E6C4) Mouse mAb with biotin-based detection (left) or SignalStain ® Boost IHC Detection Reagent (HRP, Mouse) (right).| Product Name | SignalStain ® Boost IHC Detection Reagent (HRP, Mouse) |
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| Antibody Type | Secondary Antibodies |
| Product description | SignalStain;®; Boost IHC Detection Reagent (HRP, Mouse) is a ready-to-use solution intended for use in immunohistochemical assays to detect mouse primary antibodies. SignalStain;®; Boost IHC Detection Reagent (HRP, Mouse) is a highly sensitive, one-step, polymer-based detection reagent that is specific for mouse IgG. It can be used to visualize targets in both paraffin-embedded and frozen tissue sections and it is compatible with all peroxidase-based substrates. |
| Clonality | Monoclonal |
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| Tested Applications | WBIPIHCIFFCChIP |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Tissue Specificity | SignalStain;®; Boost IHC Detection Reagent (HRP, Mouse) can be used to detect mouse primary antibodies in immunohistochemical assays. Some antibodies may require titration when used with this highly sensitive reagent. |
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Application
Immunohistochemical analysis of paraffin-embedded human seminoma using Nanog (1E6C4) Mouse mAb with biotin-based detection (left) or SignalStain ® Boost IHC Detection Reagent (HRP, Mouse) (right).| Storage Instructions | Equilibrate SignalStain;®; Boost IHC Detection Reagent (HRP, Mouse) to room temperature prior to applying to the slides. Cover sections with 1-3 drops (40-120 µl), as needed, and incubate in a humidified chamber for 30 minutes at room temperature. Wash slides and develop with any peroxidase-based substrate.; ;Storage: ; SignalStain;®; Boost IHC Detection Reagent (HRP, Mouse) should be stored at 4°C and is stable for 12 months. |
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| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
