Application
Confocal immunofluorescent analysis of mitotic HeLa cells using β-Tubulin (9F3) Rabbit mAb detected with Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) (blue) and Phospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb (Alexa Fluor ® 555 Conjugate) (red).Application
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin and U0126 (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP ® Rabbit mAb detected with Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) compared to a nonspecific negative control antibody (red).| Product Name | Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) |
|---|---|
| Antibody Type | Secondary Antibodies |
| Product description | Anti-rabbit IgG (H+L) F(ab');2; Fragment was conjugated to Alexa Fluor;®; 647 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab');2; fragment results in less non-specific binding to cells through Fc receptors. |
| Clonality | Monoclonal |
|---|---|
| Tested Applications | WBIPIHCIFFCChIP |
| Species Reactivity | Rabbit |
| Concentration | 1mg/ml |
| Tissue Specificity | F(ab');2; fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins and purified human paraproteins. |
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Application
Confocal immunofluorescent analysis of mitotic HeLa cells using β-Tubulin (9F3) Rabbit mAb detected with Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) (blue) and Phospho-Histone H3 (Ser10) (D2C8) XP ® Rabbit mAb (Alexa Fluor ® 555 Conjugate) (red).
Application
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin and U0126 (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP ® Rabbit mAb detected with Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) compared to a nonspecific negative control antibody (red).| Storage Instructions | The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution shown below should yield acceptable results for immunofluorescent and flow cytometry assays:;Immunofluorescent: 1:500 - 1:2000;Flow Cytometry: 1:250 - 1:1000; ;Storage: ; Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. ;Do not aliquot the antibody. Protect from light. Do not freeze. |
|---|---|
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
