| Product Name | Leptin tA Rat |
|---|---|
| Product description | Leptin Antagonist Triple Mutant Rat Recombinant is a singly non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, Leptin was mutated, resulting in L39A/D40A/F41A mutant. Leptin Antagonist Triple Mutant Rat Recombinant was purified by proprietary chromatographic techniques. |
| Source | Escherichia coli. |
| Sequence Similarities | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln. |
| Storage Instructions | Lyophilized Leptin-Antagonist Triple Mutant Rat Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 Leptin mutant mg/ml and up to 2 mM and filter sterilization LEP-tA mutant can be stored at 4°C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. |
| Storage Buffer | The protein was lyophilized from a concentrated (0.85mg/ml) solution with 0.003mM NaHCO3. |
