| Product Name | Human Vaspin ELISA Kit |
|---|---|
| Product description | Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.Small volumes of Vaspin elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. |
| Species Reactivity | Human |
| Sequence Similarities | Diabetes & Obesity; Immunology; Endocrinology ; Hormones; Research Assays; Assay Categories |
| Tissue Specificity | Specificity of the employed polyclonal antibodies was investigated by two different methods. Proteins of a native serum sample were separated by size exclusion chromatography, resulting fractions were tested a) by the ELISA and b) fractions positive in the ELISA were also tested by western blot. Figure 2 demonstrates that the ELISA detects a signal at about 37 kDa which is confirmed by western blot. Theoretically Vaspin has a molecular weight of 45.2 kDa. In comparison with recombinant Vaspin the signal of the SEC peak fraction is of the same size. Differences of the calculated molecular weight might result of the variability in column calibration as well as of the SEC conditions, like fraction size and flow velocity. |
| Storage Instructions | 2 - 8 degree C |
