Application
Fig1: ICC staining Vimentin(EM0401)(1/200) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ARITC-conjugatedoat anti-mouse IgG&L (HA1017) (1/100)as used as the secondary antibody. The result showed cytoplasm staining on Hela cells.Application
Fig2: IF analysis of 4% formalin-fixed, 0.2% Triton X-100 permeabilized rat brain tissue labeling GFAP with (EM140707) at 1/500 dilution, followed by Goat Anti-mouse IgG H&L (TRITC) (HA1017) secondary antibody at 1/100 dilution (red). The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1017 at 1/100 dilution.Application
Fig3: Flow cytometric analysis of Hela cells with Vimentin(EM0401) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1017 at 1/100 dilution.| Product Name | Goat Anti-Mouse IgG H&L (TRITC) |
|---|---|
| Antibody Type | Secondary Antibodies |
| Product description | This product is goat anti-mouse IgG secondary antibody labeled NHS-Rhodamine, can be used in ICC. |
| Immunogen | Mouse IgG1 |
| Conjugation | TRITC |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Goat |
| Tested Applications | ICC/IFFC |
| ICC/IF:1:50-1:200 FC:1:50-1:200 |
|
| Species Reactivity | Mouse |
| Concentration | 2mg/ml |
Application
Fig1: ICC staining Vimentin(EM0401)(1/200) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ARITC-conjugatedoat anti-mouse IgG&L (HA1017) (1/100)as used as the secondary antibody. The result showed cytoplasm staining on Hela cells.
Application
Fig2: IF analysis of 4% formalin-fixed, 0.2% Triton X-100 permeabilized rat brain tissue labeling GFAP with (EM140707) at 1/500 dilution, followed by Goat Anti-mouse IgG H&L (TRITC) (HA1017) secondary antibody at 1/100 dilution (red). The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1017 at 1/100 dilution.
Application
Fig3: Flow cytometric analysis of Hela cells with Vimentin(EM0401) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1017 at 1/100 dilution.| Application Notes | ICC/IF:1:50-1:200 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
