WB
Western blot analysis of Doublecortin on different lysates with Rat anti-Doublecortin antibody at 1/5,000 dilution. Lane 1: SH-SY5Y cell lysate, Lane 2: A549 cell lysate (negative), Lane 3: Mouse brain (P0) tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody at 1/5,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rat anti-Doublecortin antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Species: Mouse, Site: Hippocampus, Sample: Frozen section, Antibody concentration: 1:500, Antigen retrieval: Not required.| Product Name | Doublecortin Recombinant Antibody- Rat IgG1 (Chimeric) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Lissencephaly (smooth brain) is an abnormality of brain development characterized by incomplete neuronal migration and a smooth cerebral surface, resulting in severe mental retardation. Genetic analysis identified two proteins that are mutated in some cases of lissencephaly, designated lissencephaly-1 protein (LIS1) and doublecortin. LIS1 shows sequence homology to β-subunits of heterotrimeric G proteins. Doublecortin contains a consensus Abl phosphorylation site, and it has some sequence homology to a predicted kinase protein. Both proteins are highly expressed in developing brain, suggesting that they may be involved in a signal transduction pathway that is crucial to brain development. |
| Immunogen | recombinant protein |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rat |
| Tested Applications | IF-FIHCWB |
| WB:1:5000 IHC:1:500 IF-F:1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | DBCN antibody Dbct antibody DC antibody DCX antibody DCX_HUMAN antibody Doublecortex antibody Doublin antibody FLJ51296 antibody Lis X antibody Lis-X antibody Lissencephalin X antibody Lissencephalin-X antibody Lissencephaly X linked antibody Lissencephaly X linked doublecortin antibody LISX antibody Neuronal migration protein doublecortin antibody OTTHUMP00000023859 antibody OTTHUMP00000023860 antibody OTTHUMP00000216315 antibody OTTHUMP00000216316 antibody SCLH antibody XLIS antibody |
|---|---|
| Molecular Weight(MW) | 41kDa(Observed band size: 41/45kDa) |
| Cellular Localization | Cytoplasm, Cell projection. |
WB
Western blot analysis of Doublecortin on different lysates with Rat anti-Doublecortin antibody at 1/5,000 dilution. Lane 1: SH-SY5Y cell lysate, Lane 2: A549 cell lysate (negative), Lane 3: Mouse brain (P0) tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody at 1/5,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rat anti-Doublecortin antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Species: Mouse, Site: Hippocampus, Sample: Frozen section, Antibody concentration: 1:500, Antigen retrieval: Not required.| Application Notes | WB:1:5000 IHC:1:500 IF-F:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
