mIHC
mIHC analysis of human breast tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Podoplanin antibody at 1/100 dilution. The immunostaining was performed with the mTSA Kit. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.| Product Name | Podoplanin Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Puromycin aminonucleoside nephrosis (PAN) is a rat model for human minimal change nephropathy. During PAN, severe proteinuria is induced that is paralleled by a reduced expression of a rat podocyte protein, named podoplanin. Podoplanin, also known as glycoprotein 38 (gp38) is a type I membrane protein. Podoplanin localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. |
| Immunogen | Synthetic peptide within Human Podoplanin aa 35-75 / 162. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | mIHC |
| mIHC:1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | Aggrus antibody Glycoprotein 36 KD antibody Glycoprotein 36 antibody gp 36 antibody GP 38 antibody GP 40 antibody gp36 antibody GP38 antibody GP40 antibody HT1A 1 antibody HT1A1 antibody hT1alpha 1 antibody hT1alpha 2 antibody hT1alpha1 antibody hT1alpha2 antibody Lung type I cell membrane associated glycoprotein antibody Lung type I cell membrane associated glycoprotein isoform a antibody Lung type I cell membrane associated glycoprotein T1A 2 antibody OTS 8 antibody OTS8 antibody OTTHUMP00000009640 antibody OTTHUMP00000044504 antibody PA2.26 antibody PA2.26 antigen antibody Pdpn antibody PDPN_HUMAN antibody Podoplanin antibody PSEC0003 antibody PSEC0025 antibody T1 alpha antibody T1 ALPHA GENE antibody T1-alpha antibody T1A 2 antibody T1A antibody TI1A antibody TIA 2 antibody TIA2 antibody |
|---|---|
| Molecular Weight(MW) | 36 kDa |
| Cellular Localization | Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane. |
| SwissProt ID | Q86YL7 |
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mIHC
mIHC analysis of human breast tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Podoplanin antibody at 1/100 dilution. The immunostaining was performed with the mTSA Kit. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.| Application Notes | mIHC:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
