Application
Fig1: Western blot analysis of Protein Kinase D2 on different lysates with Rabbit anti-Protein Kinase D2 antibody at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate, Lane 2: HAP1-Protein Kinase D2 KD cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 120 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.Application
Fig2: ICC staining Protein Kinase D2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Protein Kinase D2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Protein Kinase D2 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of SH-SY-5Y cells with Protein Kinase D2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Protein Kinase D2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human Protein Kinase D2 aa 181-306 / 878. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:1000-1:2000 ICC/IF:1:50-1:100 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PRKD2 |
|---|---|
| Gene Synonyms | PKD2 HSPC187 nPKC-D2 |
| Gene Full Name | protein kinase D2 |
| Gene Summary | The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 97kDa(Observed band size: 105kDa) |
| Cellular Localization | Membrane. Cytoplasm. Nucleus. |
| SwissProt ID | Q9BZL6 |
|---|
Application
Fig1: Western blot analysis of Protein Kinase D2 on different lysates with Rabbit anti-Protein Kinase D2 antibody at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate, Lane 2: HAP1-Protein Kinase D2 KD cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 120 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Application
Fig2: ICC staining Protein Kinase D2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Protein Kinase D2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Protein Kinase D2 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of SH-SY-5Y cells with Protein Kinase D2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A549, SH-SY-5Y, human tonsil tissue. |
|---|---|
| Application Notes | WB:1:1000-1:2000 ICC/IF:1:50-1:100 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
