WB
Western blot analysis of Rab4A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate. Lane 2: 293T cell lysate. Lane 3: COS-1 cell lysate.ICC/IF
ICC staining of Rab4A in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).| Product Name | Rab4A Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Rab4A aa 169-218 / 218. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500 ICC/IF:1:50 |
|
| Species Reactivity | HumanMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RAB4A |
|---|---|
| Gene Synonyms | RAB4 HRES1 HRES-1 HRES-1/RAB4 |
| Gene Full Name | RAB4A, member RAS oncogene family |
| Gene Summary | This gene is a member of the largest group in the Ras superfamily of small GTPases, which regulate membrane trafficking. The encoded protein is associated with early endosomes and is involved in their sorting and recycling. The protein also plays a role in regulating the recycling of receptors from endosomes to the plasma membrane. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2012] |
| Alternative Names | HRES 1 / RAB4 antibody Oncogene RAB4 antibody Rab 4 antibody RAB 4A antibody RAB4 member RAS oncogene family antibody Rab4a antibody RAB4A member RAS oncogene family antibody RAB4A_HUMAN antibody Ras related protein Rab 4A antibody Ras related protein Rab4A antibody Ras-related protein Rab-4A antibody |
| Molecular Weight(MW) | 24 kDa |
| Cellular Localization | Cytoplasm, Membrane |
| SwissProt ID | P20338 |
|---|
WB
Western blot analysis of Rab4A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate. Lane 2: 293T cell lysate. Lane 3: COS-1 cell lysate.
ICC/IF
ICC staining of Rab4A in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:500 ICC/IF:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
