WB
Western blot analysis of Phospho-HSF1 (S326) on different lysates with Rabbit anti-Phospho-HSF1 (S326) antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Phospho-HSF1 (S326) antibody at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
Phospho-HSF1 (S326) was immunoprecipitated from 0.2 mg HeLa cell lysate with Phospho-HSF1 (S326) Recombinant Rabbit Monoclonal Antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Phospho-HSF1 (S326) Recombinant Rabbit Monoclonal Antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP, VHH(HRP) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input). Lane 2: Phospho-HSF1 (S326) Recombinant Rabbit Monoclonal Antibody IP in HeLa cell lysate. Lane 3: Rabbit IgG instead of Phospho-HSF1 (S326) Recombinant Rabbit Monoclonal Antibody in HeLa cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes.| Product Name | Phospho-HSF1 (S326) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser326 of Human HSF1 aa 301-350 / 529. |
| Modification | p-S326 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:1000-1:2000 IHC:1:50-1:200 IP:1-2μg/sample |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HSF1 |
|---|---|
| Gene Synonyms | HSTF1 |
| Gene Full Name | heat shock transcription factor 1 |
| Gene Summary | The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Jul 2017] |
| Alternative Names | Heat shock factor 1 antibody Heat shock factor protein 1 antibody Heat shock transcription factor 1 antibody HSF 1 antibody hsf1 antibody HSF1_HUMAN antibody HSTF 1 antibody HSTF1 antibody |
| Molecular Weight(MW) | 57 kDa(Observed band size: 80 kDa) |
| Cellular Localization | Cytoplasm, Nucleus. |
| SwissProt ID | Q00613 |
|---|
WB
Western blot analysis of Phospho-HSF1 (S326) on different lysates with Rabbit anti-Phospho-HSF1 (S326) antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Phospho-HSF1 (S326) antibody at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
Phospho-HSF1 (S326) was immunoprecipitated from 0.2 mg HeLa cell lysate with Phospho-HSF1 (S326) Recombinant Rabbit Monoclonal Antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Phospho-HSF1 (S326) Recombinant Rabbit Monoclonal Antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP, VHH(HRP) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input). Lane 2: Phospho-HSF1 (S326) Recombinant Rabbit Monoclonal Antibody IP in HeLa cell lysate. Lane 3: Rabbit IgG instead of Phospho-HSF1 (S326) Recombinant Rabbit Monoclonal Antibody in HeLa cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes.| Application Notes | WB:1:1000-1:2000 IHC:1:50-1:200 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
