WB
Western blot analysis of extracts of normal 293T cells, 293T transfected with SCRN1 Protein , using HRP conjugated Mouse anti HA-Tag mAb at 1:5000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.WB
Western blot analysis of extracts of normal 293T and 293F cells, 293T transfected with CD2 Protein and HA-N Protein ,293F transfected with CD95-N Protein using HRP-conjugated Mouse anti HA-Tag mAb at 1:5000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.| Product Name | HRP-conjugated Mouse anti HA-Tag mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | HA tag recombinant protein. |
| Immunogen | Synthetic peptide. |
| Conjugation | HRP |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:5000 |
|
| Species Reactivity | All |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | HA epitope tag antibody HA1 antibody HA2 antibody hemagglutinin antibody Hemagglutinin HA1 chain antibody Hemagglutinin HA2 chain antibody |
|---|---|
| Function | Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification. |
WB
Western blot analysis of extracts of normal 293T cells, 293T transfected with SCRN1 Protein , using HRP conjugated Mouse anti HA-Tag mAb at 1:5000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
WB
Western blot analysis of extracts of normal 293T and 293F cells, 293T transfected with CD2 Protein and HA-N Protein ,293F transfected with CD95-N Protein using HRP-conjugated Mouse anti HA-Tag mAb at 1:5000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.| Application Notes | WB:1:500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
