WB
Western blot analysis of HMGCR on different lysates with Rabbit anti-HMGCR antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate, Lane 2: THP-1 cell lysate, Lane 3: HepG2 cell lysate, Lane 4: Mouse testis tissue lysate, Lane 5: Rat testis tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-HMGCR antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
HMGCR was immunoprecipitated in 0.2mg HeLa cell lysate with Rabbit anti-HMGCR antibody at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using Rabbit anti-HMGCR antibody at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2: Rabbit IgG instead of Rabbit anti-HMGCR antibody in HeLa cell lysate, Lane 3: Rabbit anti-HMGCR antibody IP in HeLa cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 43 seconds.| Product Name | HMGCR Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The human enzyme hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) limits the rate of cholesterol synthesis, a necessary process for cellular growth, in liver tissue. Phosphorylation of HMGCR inactivates the enzyme, which occurs via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from the product of the reductase reaction. Inhibitors of HMGCR (statins) exert anti-inflammatory effects and decrease the frequency of cardiovascular events by lowering plasma cholesterol. Additionally, intermediate products along the pathway catalyzed by HMGCR, which modulate signal transducing proteins such as Ras, provide possible ties between HMGCR regulation and new chemotherapeutic methods. |
| Immunogen | Synthetic peptide within Human HMGCR aa 438-489 / 888. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IHCIPWB |
| WB:1:5000 IHC:1:100 IP:Use at an assay dependent concentration. |
|
| Species Reactivity | HumanMouseRatZebrafish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HMGCR |
|---|---|
| Gene Synonyms | MYPLG LDLCQ3 LGMDR28 |
| Gene Full Name | 3-hydroxy-3-methylglutaryl-CoA reductase |
| Gene Summary | HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
| Molecular Weight(MW) | 97kDa（Observed band size: 100kDa） |
| Cellular Localization | Endoplasmic reticulum membrane. |
WB
Western blot analysis of HMGCR on different lysates with Rabbit anti-HMGCR antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate, Lane 2: THP-1 cell lysate, Lane 3: HepG2 cell lysate, Lane 4: Mouse testis tissue lysate, Lane 5: Rat testis tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-HMGCR antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
HMGCR was immunoprecipitated in 0.2mg HeLa cell lysate with Rabbit anti-HMGCR antibody at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using Rabbit anti-HMGCR antibody at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2: Rabbit IgG instead of Rabbit anti-HMGCR antibody in HeLa cell lysate, Lane 3: Rabbit anti-HMGCR antibody IP in HeLa cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 43 seconds.| Application Notes | WB:1:5000 IHC:1:100 IP:Use at an assay dependent concentration. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
