WB
Western blot analysis of alpha sarcoglycan on different lysates with Rabbit anti-alpha sarcoglycan antibody at 1/1,000 dilution. Lane 1: Mouse heart tissue lysate, Lane 2: Mouse skeletal muscle tissue lysate, Lane 3: Rat heart tissue lysate, Lane 4: Rat skeletal muscle tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-alpha sarcoglycan antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-alpha sarcoglycan antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-alpha sarcoglycan antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
alpha sarcoglycan was immunoprecipitated from 0.2 mg mouse heart tissue lysate with Rabbit anti-alpha sarcoglycan antibody at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using Rabbit anti-alpha sarcoglycan antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse heart tissue lysate (input), Lane 2: Rabbit anti-alpha sarcoglycan antibody IP in mouse heart tissue lysate, Lane 3: Rabbit IgG instead of Rabbit anti-alpha sarcoglycan antibody in mouse heart tissue lysate. Blocking/Dilution buffer: 5% NFDM/TBST. Exposure time: 30 seconds.| Product Name | alpha sarcoglycan Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human alpha sarcoglycan aa 338-387 / 387. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IHCIPWB |
| WB:1:1000 IHC:1:200-1:1000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SGCA |
|---|---|
| Gene Synonyms | ADL DAG2 50DAG DMDA2 LGMD2D LGMDR3 SCARMD1 adhalin |
| Gene Full Name | sarcoglycan alpha |
| Gene Summary | This gene encodes a component of the dystrophin-glycoprotein complex (DGC), which is critical to the stability of muscle fiber membranes and to the linking of the actin cytoskeleton to the extracellular matrix. Its expression is thought to be restricted to striated muscle. Mutations in this gene result in type 2D autosomal recessive limb-girdle muscular dystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008] |
| Molecular Weight(MW) | 43kDa(Observed band size: 50kDa) |
| Cellular Localization | Sarcolemma, cytoskeleton. |
WB
Western blot analysis of alpha sarcoglycan on different lysates with Rabbit anti-alpha sarcoglycan antibody at 1/1,000 dilution. Lane 1: Mouse heart tissue lysate, Lane 2: Mouse skeletal muscle tissue lysate, Lane 3: Rat heart tissue lysate, Lane 4: Rat skeletal muscle tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-alpha sarcoglycan antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-alpha sarcoglycan antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-alpha sarcoglycan antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
alpha sarcoglycan was immunoprecipitated from 0.2 mg mouse heart tissue lysate with Rabbit anti-alpha sarcoglycan antibody at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using Rabbit anti-alpha sarcoglycan antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse heart tissue lysate (input), Lane 2: Rabbit anti-alpha sarcoglycan antibody IP in mouse heart tissue lysate, Lane 3: Rabbit IgG instead of Rabbit anti-alpha sarcoglycan antibody in mouse heart tissue lysate. Blocking/Dilution buffer: 5% NFDM/TBST. Exposure time: 30 seconds.| Application Notes | WB:1:1000 IHC:1:200-1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
