WB
Western blot analysis of PDK1 on different lysates with Rabbit anti-PDK1 antibody at 1/5,000 dilution. Lane 1: HepG2 cell lysate (15 µg/Lane), Lane 2: HEK-293 cell lysate (15 µg/Lane), Lane 3: LNCaP cell lysate (15 µg/Lane), Lane 4: NIH/3T3 cell lysate (15 µg/Lane), Lane 5: PC-12 cell lysate (15 µg/Lane), Lane 6: Mouse heart tissue lysate (30 µg/Lane), Lane 7: Rat heart tissue lysate (30 µg/Lane), Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-PDK1 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-PDK1 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
PDK1 was immunoprecipitated from 0.2 mg mouse heart tissue lysate with Rabbit anti-PDK1 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-PDK1 antibody at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse heart tissue lysate (input), Lane 2: Rabbit anti-PDK1 antibody IP in mouse heart tissue lysate, Lane 3: Rabbit IgG instead of Rabbit anti-PDK1 antibody in mouse heart tissue lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 3 minutes.| Product Name | PDK1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mitochondrial pyruvate dehydrogenase (PDH) catalyzes the oxidative decarboxylation of pyruvate and plays a central role in the regulation of homeostasis of carbohydrate fuels in mammals. PDH activity is controlled by a phosphorylation/dephosphorylation cycle, phosphorylation leading to inactivation and dephosphorylation leading to reactivation of PDH. The phosphorylation of PDH is catalyzed by pyruvate dehydrogenase kinase (PDK), the activity of which is stimulated by the products of PDH catalysis. PDK1 consists of alpha and beta subunits; the kinase activity resides in the alpha subunit. Three PDK isoenzymes have been identified in humans (PDK1, 2 and 3) and two have been identified in rodent (PDK1 and 2). |
| Immunogen | Synthetic peptide within Human PDK1 aa 16-65 / 436. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IHCIPWB |
| WB:1:5000 IHC:1:2000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PDK1 |
|---|---|
| Gene Full Name | pyruvate dehydrogenase kinase 1 |
| Gene Summary | Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013] |
| Molecular Weight(MW) | 49 kDa(Observed band size: 45 kDa) |
| Cellular Localization | Mitochondrion matrix. |
WB
Western blot analysis of PDK1 on different lysates with Rabbit anti-PDK1 antibody at 1/5,000 dilution. Lane 1: HepG2 cell lysate (15 µg/Lane), Lane 2: HEK-293 cell lysate (15 µg/Lane), Lane 3: LNCaP cell lysate (15 µg/Lane), Lane 4: NIH/3T3 cell lysate (15 µg/Lane), Lane 5: PC-12 cell lysate (15 µg/Lane), Lane 6: Mouse heart tissue lysate (30 µg/Lane), Lane 7: Rat heart tissue lysate (30 µg/Lane), Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-PDK1 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-PDK1 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
PDK1 was immunoprecipitated from 0.2 mg mouse heart tissue lysate with Rabbit anti-PDK1 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-PDK1 antibody at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse heart tissue lysate (input), Lane 2: Rabbit anti-PDK1 antibody IP in mouse heart tissue lysate, Lane 3: Rabbit IgG instead of Rabbit anti-PDK1 antibody in mouse heart tissue lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 3 minutes.| Application Notes | WB:1:5000 IHC:1:2000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
