WB
Western blot analysis of FHL2 on different lysates with Rabbit anti-FHL2 antibody at 1/1,000 dilution. Lane 1: Rat heart tissue lysate, Lane 2: Mouse heart tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-FHL2 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded human fetal heart tissue using anti-FHL2 antibody. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-FHL2 antibody. Counter stained with hematoxylin.| Product Name | FHL2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The four-and-a-half-LIM domain (FHL) proteins include FHL-1 (SLIM1), FHL-2 (SLIM3), FHL-3 (SLIM2) and FHL-4. The signature "half-domain", a single zinc finger domain located in the N-terminal region, differentiates FHLs from other LIM-only proteins, which have numbers of zinc fingers. Specific combinations of FHL proteins elicit selective activation of both CREB and CREM. Skeletal and cardiac muscle express FHL-1 in high levels as compared to the low level of expression in smooth muscle of the colon, small intestine and prostate. FHL-1 localizes to the cytosol of myoblasts, myotubes, and differentiated myocytes. FHL-2 is also located in cardiac and skeletal muscle, as well as in placenta and ovary tissues. FHL-3 is found in skeletal muscle, but absent in cardiac muscle. FHL-4 is expressed exclusively by the seminiferous epithelium of the testis, which suggests that FHL-4 is involved in spermatogenesis. The genetic loci for FHLs vary considerably despite similiar amino acid sequences among the FHL group. |
| Immunogen | Recombinant protein within Human FHL2 aa 30-280 / 279. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | AAG 11 antibody AAG11 antibody Aging associated gene 11 antibody Down regulated in rhabdomyosarcoma LIM protein antibody Downregulated in rhabdomyosarcoma LIM protein antibody DRAL antibody FHL 2 antibody FHL-2 antibody Fhl2 antibody FHL2 protein antibody FHL2_HUMAN antibody Four and a half LIM domain protein 2 antibody Four and a half LIM domains 2 antibody Four and a half LIM domains protein 2 antibody KIAA0990 antibody LIM domain protein DRAL antibody Skeletal muscle LIM protein 3 antibody Skeletal muscle LIM-protein 3 antibody SLIM 3 antibody SLIM-3 antibody SLIM3 antibody |
|---|---|
| Molecular Weight(MW) | 32 kDa(Observed band size: 30 kDa) |
| Cellular Localization | Cytoplasm. Nucleus. |
WB
Western blot analysis of FHL2 on different lysates with Rabbit anti-FHL2 antibody at 1/1,000 dilution. Lane 1: Rat heart tissue lysate, Lane 2: Mouse heart tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-FHL2 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded human fetal heart tissue using anti-FHL2 antibody. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-FHL2 antibody. Counter stained with hematoxylin.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
