WB
Western blot analysis of Sarcomeric Alpha Actinin on different lysates with Rabbit anti-Sarcomeric Alpha Actinin antibody at 1/1,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate, Lane 2: Mouse heart tissue lysate, Lane 3: Rat heart tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue with Rabbit anti-Sarcomeric Alpha Actinin antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Sarcomeric Alpha Actinin Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human Sarcomeric Alpha Actinin aa 1-150. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | IHCWB |
| WB:1:500 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ACTN2 |
|---|---|
| Gene Synonyms | MPD6 CMH23 CMYO8 CMYP8 CMD1AA MYOCOZ |
| Gene Full Name | actinin alpha 2 |
| Gene Summary | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013] |
| Alternative Names | Actin binding protein antibody Actinin alpha 2 antibody ACTN 2 antibody ACTN2 antibody ACTN2_HUMAN antibody Alpha actinin 2 antibody Alpha actinin skeletal muscle antibody Alpha actinin skeletal muscle isoform 2 antibody Alpha-actinin skeletal muscle isoform 2 antibody Alpha-actinin-2 antibody CMD1AA antibody F actin cross linking protein antibody F-actin cross-linking protein antibody |
| Molecular Weight(MW) | 104kDa |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of Sarcomeric Alpha Actinin on different lysates with Rabbit anti-Sarcomeric Alpha Actinin antibody at 1/1,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate, Lane 2: Mouse heart tissue lysate, Lane 3: Rat heart tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue with Rabbit anti-Sarcomeric Alpha Actinin antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Positive Control | Mouse heart tissue, rat heart tissue, human colon tissue, human fetal skeletal muscle tissue. |
|---|---|
| Application Notes | WB:1:500 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
