Application
Fig1: Western blot analysis of MAP LC3β expression in non-transfected 293T (A), human MAP LC3β transfected 293T (B) and IM R-32 (C) whole cell lysates. .Application
Fig2： Western blot analysis of MAP LC3β expression in non-transfected (A) and human MAP LC3β transfected (B) 293T whole cell lysates and mouse brain tissue extract (C). .| Product Name | LC3B |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATG8F antibody Autophagy-related protein LC3 B antibody Autophagy-related ubiquitin-like modifier LC3 B antibody LC3B antibody LC3II antibody MAP1 light chain 3 like protein 2 antibody MAP1 light chain 3-like protein 2 antibody MAP1A/1BLC3 antibody MAP1A/MAP1B LC3 B antibody MAP1A/MAP1B light chain 3 B antibody MAP1ALC3 antibody MAP1LC3B a antibody Map1lc3b antibody Microtubule associated protein 1 light chain 3 beta antibody Microtubule-associated protein 1 light chain 3 beta antibody Microtubule-associated proteins 1A/1B light chain 3B antibody MLP3B_HUMAN antibody |
| Product description | Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP-light chain 3 beta (MAP-LC3β ) and MAP-light chain 3 alpha (MAP-LC3α) are subunits of both MAP1A and MAP1B. MAP-LC3β , a homolog of Apg8p, is essential for autophagy and associated to the autophagosome membranes after processing. Two forms of LC3β , the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3β , followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3’ untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. MAP LC3β may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle. |
| Immunogen | peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | WBIPIF |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ATG8F antibody Autophagy-related protein LC3 B antibody Autophagy-related ubiquitin-like modifier LC3 B antibody LC3B antibody LC3II antibody MAP1 light chain 3 like protein 2 antibody MAP1 light chain 3-like protein 2 antibody MAP1A/1BLC3 antibody MAP1A/MAP1B LC3 B antibody MAP1A/MAP1B light chain 3 B antibody MAP1ALC3 antibody MAP1LC3B a antibody Map1lc3b antibody Microtubule associated protein 1 light chain 3 beta antibody Microtubule-associated protein 1 light chain 3 beta antibody Microtubule-associated proteins 1A/1B light chain 3B antibody MLP3B_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 15 kDa |
| Cellular Localization | Cytoplasm, Endomembrane system Cytoplasmic vesicle |
| SwissProt ID | Q9GZQ8 |
|---|
Application
Fig1: Western blot analysis of MAP LC3β expression in non-transfected 293T (A), human MAP LC3β transfected 293T (B) and IM R-32 (C) whole cell lysates. .
Application
Fig2： Western blot analysis of MAP LC3β expression in non-transfected (A) and human MAP LC3β transfected (B) 293T whole cell lysates and mouse brain tissue extract (C). .| Positive Control | 293T, IM R-32, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
