Application
Fig1: Western blot analysis of phosphorylated-IκB-α expression in Jurkat (A) and TNFα /ALLN-treated Jurkat (B) whole cell lysates.| Product Name | Phospho-I-kappa-B-alpha (Ser 32) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | I kappa B alpha antibody I-kappa-B-alpha antibody IkappaBalpha antibody IkB-alpha antibody IKBA antibody IKBA_HUMAN antibody IKBalpha antibody MAD 3 antibody MAD3 antibody Major histocompatibility complex enhancer-binding protein MAD3 antibody NF kappa B inhibitor alpha antibody NF-kappa-B inhibitor alpha antibody NFKBI antibody NFKBIA antibody Nuclear factor of kappa light chain gene enhancer in B cells antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody |
| Product description | On the basis of both functional and structural considerations, members of the IκB family of proteins can be divided into four groups. The first of these groups, IκB-α, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFκB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to κB sites, suggesting that the IκB-α family binds to the p65 subunit of p50-p65 heterocomplex through Ankyrin repeats. The second member of the IκB family is represented by a protein designated IκB-β. The third group of IκB proteins is represented by IκB-γ, a protein identical in sequence with the C-terminal domain of the p110 precursor of NFκB p50 and expressed predominantly in lymphoid cells. An additional IκB family member has been identified as IκB-ε, a protein which has several phosphorylated forms and is primarily found complexed with RelA and/or c-Rel. |
| Immunogen | A short amino acid sequence containing Ser 32 phosphorylated IκB-α of human origin. |
| Modification | p-Ser 32 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | WBIPIF |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | I kappa B alpha antibody
I-kappa-B-alpha antibody IkappaBalpha antibody IkB-alpha antibody IKBA antibody IKBA_HUMAN antibody IKBalpha antibody MAD 3 antibody MAD3 antibody Major histocompatibility complex enhancer-binding protein MAD3 antibody NF kappa B inhibitor alpha antibody NF-kappa-B inhibitor alpha antibody NFKBI antibody NFKBIA antibody Nuclear factor of kappa light chain gene enhancer in B cells antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody |
|---|---|
| Molecular Weight(MW) | 41kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | P25963 |
|---|
Application
Fig1: Western blot analysis of phosphorylated-IκB-α expression in Jurkat (A) and TNFα /ALLN-treated Jurkat (B) whole cell lysates.| Positive Control | Jurkat. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
